Hi guys. hope you all r doing great. I have a little trouble with my prism type TIRF set up. I would be greatful if you guys can suggest something. We get a lot of concentric fringes around the point of illumination and we cant get enough area of uniform illumination with bright enough intensity to image. Did anyone else got into something similar? Highly appreciate a reply.
Erstwhile biological physicist cum big-data wrangler. I seek interesting problems to solve, and apply my crushing intellect to make them submit to my will. I use whatever tools the job requires: I learn new programming languages and paradigms for breakfast. I am a relentless optimizer, of code, of process, and of myself. I like doing things I'm good at, and like becoming good at doing new things.
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Hi guys. hope you all r doing great. I have a little trouble with my prism type TIRF set up. I would be greatful if you guys can suggest something. We get a lot of concentric fringes around the point of illumination and we cant get enough area of uniform illumination with bright enough intensity to image. Did anyone else got into something similar? Highly appreciate a reply.
People are still doing prism type TIRF? Ew.
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