Hi guys. hope you all r doing great. I have a little trouble with my prism type TIRF set up. I would be greatful if you guys can suggest something. We get a lot of concentric fringes around the point of illumination and we cant get enough area of uniform illumination with bright enough intensity to image. Did anyone else got into something similar? Highly appreciate a reply.
I'm a postdoctoral researcher in the Block Lab at Stanford University. I'm interested in optical tweezers, single molecule fluorescence, and RNA structure and dynamics. You can reach me at mpgordon AT NO SPAM stanford.edu. My CV can be found here.
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Hi guys. hope you all r doing great. I have a little trouble with my prism type TIRF set up. I would be greatful if you guys can suggest something. We get a lot of concentric fringes around the point of illumination and we cant get enough area of uniform illumination with bright enough intensity to image. Did anyone else got into something similar? Highly appreciate a reply.
People are still doing prism type TIRF? Ew.
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