- The talk at imaging and spectroscopy on mNeptune looks like it will be incredibly useful. It's a new fluorescent protein that just sneaks into the water window. You can excite it with 633 nm, and detect it at 650, allowing you to do intravital imaging while avoiding hemoglobin absoprtion. I suspect many people will find that very useful.
- Two different talks on superresolution imaging of neurons are starting to show the real promise of superresolution imaging. The attempt to use it to trace all the neurons in a brain sample looks very ambitious, and I appreciate ambitious. Of course, the problem is it's sloooowwwww. If you have to fix the cell, why can't you use EM? That's a genuine question, by the way. Why can't you just do EM on brain slices and trace neurons that way, with way more resolution than you can get with any fluorescence technique? There must be a reason this doesn't work. Are they hard to stain with EM probes? Do they degrade rapidly under EM? Inquiring minds want to know.
Monday, February 22, 2010
On a more positive note
I'm a bit of a curmudgeon (actually, I'm a lot of a curmudgeon) so it's easy for me to complain, and as a postscript, I thought I'd say some nice things: